Differential Gene Expression Analysis

This is a reproduction of the RNA-Seq data analysis performed by Himes et al. in order to identify transcriptional changes in Human Airway Smooth Muscle (HASM) due to asthma treatment with dexamethasone.

Tasks performed:

  • RNA-Seq data prefiltering and exploration.
  • Differential analysis using DESeq2.
  • Visualization of differentially expressed genes through volcano plots and heatmaps.
  • GO enrichment analysis.

The code for this project can be found here.